Rickettsia rickettsia and felis/typhi PCR Panel – Whole Blood

The PCR-based diagnostic assay for detection of Rickettsia from blood is highly specific to R. rickettsia and R. felis/typhi DNAs. The combination of the following three steps imparts a very high specificity and sensitivity to the test.

  • Hybridization/Selection
  • Amplification of Ehrlichia-specific DNA
  • Detection of Ehrlichia-specific amplified DNA fragments

Result Interpretation

Rejection Criteria Refer to general collection procedure

Reference Range

R. rickettsii: Negative, R. rickettsii specific DNA was not detected

R. felis/typhi: Negative, R. felis/typhi specific DNA was not detected

Clinical Significance

The Rickettsia species PCR test is an assay that detects Rickettsia species-specific DNA in clinical samples. Rickettsia 17 kDa antigen gene fragments are hybrid-selected by probes, followed by PCR amplification of the selected DNA fragment. PCR products are tested with Rickettsia specific probes (R. rickettsii and R.felis/typhi) by Southern Blot Assays. The primers and probes used for the selection of Rickettsia gene fragment encoding the 17 kDa antigen DNA fragments are designed from published sequences.

Limitations

  1. This test should only be performed in conjunction with Southern Blot.
  2. Results should be interpreted in conjunction with other laboratory and clinical findings.
  3. Test results can only help the physician in confirming clinical diagnosis.

Special Instructions Only R. rickettsii PCR results will be reported to NY residents. Test 998 is included in panel 995.

Ordering Information

Test Number

Sample Type

Minimum Volume

Container

998

EDTA Whole Blood

2ml

EDTA lavender top tube

986

Cerebral Spinal Fluid

2ml

Sterile leak-proof tube

Collection and Shipping Instructions

Test 998: See Serum and Whole Blood Samples- Collection & Shipping Instructions

Test 986: See Miscellaneous Specimen Collection & Shipping Instructions

Test Offered

Test# 998 Rickettsia rickettsia and felis/typhi PCR Panel – Whole Blood (only R. rickettsia will be reported for NY residents**) CPT Code 87797, 87798 x2
Test# 986 Rickettsia rickettsia and felis/typhi PCR Panel – CSF (only R. rickettsia will be reported for NY residents**) CPT Code 87797, 87798 x2
Rickettsia rickettsia and felis/typhi PCR Panel – Whole Blood is part of the following Test Panels:
Test Panel# 995 Complete Rickettsia Panel