Lyme Dot-blot Assay (LDA) is a qualitative immunoassay for the direct detection of Borrelia burgdorferi specific antigens in urine using anti-B. burgdorferi antibodies.

Result Interpretation

Reference Range Negative: No B. burgdorferi antigens detected

Clinical Significance

The Lyme Dot-Blot Assay (LDA) is a qualitative immunoassay for the direct detection of Borrelia burgdorferi specific antigens in urine or cerebral spinal fluid using B. burgdorferi antibodies. The antigens present in urine or CSF are immobilized onto a membrane in a Dot-blot format and are incubated with anti-B. burgdorferi specific antibodies. After washing, the bound anti-B. burgdorferi specific antibodies are reacted with anti-rabbit IgG, which is visualized by an enzyme/substrate reaction.

If the initial Lyme panel tests on blood samples are negative, including PCR, but symptoms for Lyme disease are present, the follow-Up Lyme Panel 875, which includes LDA on urine and PCR, can be helpful in making the diagnosis.


  1. Negative tests do not exclude diagnosis of Lyme disease
  2. Cross-reactions with Leptospira may occur.
  3. False positive may occur for patients with a Urinary Tract Infection (UTI), currently being treated for a UTI, or who have undergone treatment for a UTI within the last two weeks.
  4. Results should be interpreted in conjunction with other laboratory and clinical findings.

Special Instructions This test is not yet available for NY residents. Test 805 is included in panel 875.

Ordering Information

Test Number

Sample Type

Minimum Volume





B/D C&S Vacutainer gray-top tube



4ml x 2

B/D C&S Vacutainer gray-top tube



4ml x 3

B/D C&S Vacutainer gray-top tube


Cerebral Spinal Fluid

2 ml

Sterile, Leak-proof tube

Collection and Shipping Instructions

Tests 800, 802, 805: See Urine Samples- Collection & Shipping Instructions

Test 810: See Miscellaneous Specimen Collection & Shipping Instructions

Test Offered

Test# 800 Lyme Dot Blot** CPT Code 87449
Test# 802 Lyme Dot Blot – 2 Samples** CPT Code 87449 x2
Test# 805 Lyme Dot Blot – 3 Samples** CPT Code 87449 x3
Test# 810 Lyme Dot Blot Assay – CSF** CPT Code 87449
Lyme DOT BLOT Assay (LDA) Urine and/or CSF are part of the following test panels:
Test Panel# 875 Urine Lyme Panel**
Test Panel# 8075 NMN Urine Panel**
Test Panel# 8085 CSF Panel**

**Test/Panels are not yet available for New York Residents