The Lyme IGXSpot is an Enzyme-Linked ImmunoSpot assay that detects human T cells reactive to B. burgdorferi specific antigens in vitro. It is well documented that both humoral and cellular immune responses develop in Borrelia infection. The cellular immune response develops much earlier than humoral response in most patients infected with B. burgdorferi. In some patients sero-conversion from cellular to humoral response does not occur or occurs much later in disease; and in some patients with chronic form of the disease, the humoral response is poor. Thus the Lyme IGXSpot test is recommended for detection of very early and/or late B. burgdorferi infection; and in seronegative patient’s whole blood samples.

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Principle

The B. burgdorferi IGXSpot is an Enzyme-Linked ImmunoSpot (ELISPOT) assay that detects human T-cells reactive to B. burgdorferi specific antigens in vitro. ELISPOT is a widely used method for detecting and monitoring cellular immune responses to specific antigens. The IGXSpot assay allows visualization of the secretory product(s) of individual activated or responding cells to B. burgdorferi specific antigens. Each spot that develops in the assay represents a single reactive cell.


Advantage

Detects specific T-cell responses soon after infection, when antibodies to the organisms are not detectable or late in the disease when the levels of antibodies are very low. When combined with Lyme ImmunoBlot tests, provides information on the full spectrum of patient’s immune response to infection and stage of disease. Especially useful for seronegative patients.


Lyme IGXSpot

Reference Range

Reference Range: >2 colonies positive.

Lyme IGXSpot

Clinical Significance

It is well documented that both humoral and cellular immune responses develop to Borrelia infection. Assessment of both the function and the frequency of Borrelia-specific T cells is crucial for evaluating the cellular immune response to, and diagnosis of Borrelia infection. Due to the clonal expansion (proliferation) of antigen-specific T cells in vivo during an immune response, the presence of increased frequencies of Borrelia antigen-specific effector/memory T cells in peripheral blood suggests prior infection/exposure to Borrelia. Because of the apparent prevalence of either humoral or cellular immunity in infected individuals, combination of the B. burgdorferi IgXSpot with Lyme serology assay would further increase the sensitivity of Lyme disease diagnosis.

Lyme IGXSpot

Limitations

For diagnostic purposes, the IgXSpot test results should be used in conjunction with clinical symptoms and other evidence available to the diagnosing physician.

Lyme IGXSpot

Ordering Information

Lyme IGXSpot

Test Offered

Test# 300

Lyme IGXSpot

CPT Code 86356