The Lyme IGXSpot is an Enzyme-Linked ImmunoSpot assay that detects human T cells reactive to B. burgdorferi specific antigens in vitro. It is well documented that both humoral and cellular immune responses develop in Borrelia infection. The cellular immune response develops much earlier than humoral response in most patients infected with B. burgdorferi. In some patients sero-conversion from cellular to humoral response does not occur or occurs much later in disease; and in some patients with chronic form of the disease, the humoral response is poor. Thus the Lyme IGXSpot test is recommended for detection of very early and/or late B. burgdorferi infection; and in seronegative patient’s whole blood samples.
The B. burgdorferi IGXSpot is an Enzyme-Linked ImmunoSpot (ELISPOT) assay that detects human T-cells reactive to B. burgdorferi specific antigens in vitro. ELISPOT is a widely used method for detecting and monitoring cellular immune responses to specific antigens. The IGXSpot assay allows visualization of the secretory product(s) of individual activated or responding cells to B. burgdorferi specific antigens. Each spot that develops in the assay represents a single reactive cell.