The Lyme ImmunoBlot is a qualitative immunoassay in which antibodies specific to the B. burgdorferi antigens on a membrane strip are visualized. It is a qualitative test and is more sensitive and specific than the ELISA, IFA and traditional Western Blot tests. In early or late stage of the disease when antibody levels are very low, ImmunoBlot can be positive whereas ELISA and IFA tests can be negative. This test must be used if the Lyme IgG/IgM antibody serology or  Lyme IgG/IgA/IgM IFA is positive or equivocal.

||  Download Data Sheet


Principle

The Lyme ImmunoBlot assay is based upon an antigen-antibody complex formation in the following steps:

  1. Binding of anti-Borrelia specific antibodies in human serum to the ImmunoBlot strip.  ImmunoBlot strip is a membrane strip with fixed B. burgdorferi recombinant antigens on it.
  2. Binding of enzyme labeled anti-human IgG or IgM antibodies to the human anti– Borrelia antibodies bound to fixed B. burgdorferi antigens on the membrane.
  3. Reaction with BCIP/NBT, a chromogenic substrate with bound antibodies on the strip.  A dark purple colored precipitate (band) develops on the antigen-antibody complexes.

Lyme ImmunoBlot IgM

The Lyme ImmunoBlot IgM is a very sensitive indicator of exposure to B. burgdorferi.  It may be positive as early as 1 week after a tick bite. This test will usually remain positive for six to eight weeks after initial exposure and in some patients will remain positive for a very long time.  Re-exposure will also cause this test to be positive for a brief period of time.  For the testing to be complete, it is preferable that the IgM ImmunoBlot be run along with the IgG ImmunoBlot.


Lyme ImmunoBlot IgG

The IgG ImmunoBlot is an immunoassay and qualitative test in which antibodies are visualized.  The IgG antibody is typically present a few months following initial infection.


Lyme ImmunoBlot IgM

Reference Range

Negative <2 stared bands present on the blot


Lyme ImmunoBlot IgG

Reference Range

Negative <2 stared bands present on the blot

Lyme ImmunoBlot IgM

Clinical Significance

The Lyme ImmunoBlot IgM is a sensitive indicator of exposure to B. burgdorferi. It may be positive as early as one week after a tick bite. This test will usually remain positive for six to eight weeks after initial exposure. Re-exposure will also cause this test to be positive for a brief period of time. In some patients this test can be positive for a very long time.  Lyme ImmunoBlot IgM must be performed on any sample with positive or equivocal result for Lyme IgG/IgM antibody serology or Lyme IgG/IgA/IgM IFA.   For the testing to be complete, it is preferable that the IgM ImmunoBlot be run along with the IgG ImmunoBlot.


Lyme ImmunoBlot IgG

Clinical Significance

The Lyme ImmunoBlot IgG is a sensitive indicator of an exposure to B. burgdorferi.  IgG antibody is typically present a few months following the initial infection. Lyme Western Blot IgG must be performed on any sample with positive or equivocal result for Lyme IgG/IgM antibody serology or Lyme IgG/IgA/IgM IFA.

Lyme ImmunoBlot IgM

Limitations

  1. Patients with other spirochetal disease and/or who test positive for rheumatoid factor or Epstein Barr virus may have cross-reacting antibodies and may have a positive result for 31, 41, and/or 83 kDa antigens.
  2. Positive results for 31 kDa antigens may be present after Lyme vaccination in uninfected persons.
  3. A negative Western Blot does not exclude the possibility of infection with B. burgdorferi.
  4. IGeneX interpretation is based on internal validation studies.
  5. The results of this test must be interpreted in relation to patient’s clinical history, epidemiological data, stages of disease, clinical symptoms or other laboratory results.

Presence of any of the following bands: 23-25, 31, 34, 39 and  83-93 kDa as indeterminate or if only one of these bands is present in a negative report, it may have clinical significance. Therefore, we recommend testing with another method and/or retesting in 4-6 weeks.


Lyme ImmunoBlot IgG

Limitations

  1. Positive results for 31 and/or 34 kDa may be present after Lyme vaccination in uninfected persons.
  2. A negative Lyme ImmunoBlot IgG does not exclude the possibility of infection with B. burgdorferi.
  3. IGeneX interpretation is based on internal validation studies.
  4. The results of this test must be interpreted in relation to patient’s clinical history, epidemiological data, stage of disease, clinical symptoms or other laboratory results.

Presence of an indeterminate number of double starred bands in a negative report may indicate clinical significance. Therefore, we recommend testing with another method and/or retesting in 4-6 weeks. 

Result Interpretation

Lyme ImmunoBlot IgM and IgG

Ordering Information

Lyme ImmunoBlot

Test Offered

Test# 325

Lyme ImmunoBlot IgM

CPT Code 86617

Test# 335

Lyme ImmunoBlot IgG          

CPT Code 86617

Lyme ImmunoBlot IgG & IgM are part of the following Test Panels:

Test Panel IB1

Lyme ImmunoBlot Panel 1**

Test Panel IB2

Lyme ImmunoBlot Panel 2**

Test Panel IB3

Lyme ImmunoBlot Panel 3**

Test Panel LTP1

Lyme/TRRF Panel 1**

Test Panel LTP2

Lyme/TBRF Panel 2**

Test Panel LTP3

Lyme/TBRF Panel 3**

**Test/Panels are not yet available for New York Residents