IGG AND IGM ANTIBODY FOR EHRLICHIA (HME AND HGA) BY INDIRECT IMMUNOFLUORESENCE ASSAYS
The Ehrlichia immuno-fluorescence assays (IFAs), Anaplasma phagocytophilum IFA (HGA IFA) and Ehrlichia chaffeensis IFA (HME IFA) are designed to detect human IgG and IgM antibodies to HGA and HME antigens respectively in human serum. For diagnostic purposes, HGA and HME IFA test results should be used in conjunction with other information available to the physician.
The Ehrlichia IFA assay is a “two-stage” sandwich assay, based upon an antigen-antibody complex formation in the following steps:
- Binding of anti-Ehrlichia specific antibodies in human serum to fixed Ehrlichia
- Binding of fluorescent-labeled anti-human IgG/IgM antibodies to the human anti– Ehrlichia antibodies bound to Ehrlichia on the slide.
- Identifing green fluorescing Ehrlichia organisms with a fluorescent microscope.x.
Note: For HGA IFA, A. phagocytophilum is fixed to the slide and for HME IFA, Ehrlichia chaffeensis is fixed on the slide.