Rickettsia rickettsii/typhi IgG Antibody
The Rickettsia immunofluorescent assay (IFA) is designed to detect Rickettsia specific antibodies in human serum. For diagnostic purposes, IFA test results should be used in conjunction with other data available to the physician.
The Rickettsia IFA assay is a “two-stage” sandwich assay, based upon an antigen-antibody complex formation in the following steps:
- Binding of anti-Rickettsia specific antibodies in human serum to fixed Rickettsia
- Binding of fluorescent-labeled anti-human IgG/IgM antibodies to the human anti– Rickettsia antibodies bound to Rickettsia on the slide.
- Identifing green fluorescing Rickettsia organisms with a fluorescent microscope.